A simple, accurate, precise, sensitive, specific and rapid HPTLC method was developed for simultaneous estimation of ethinyl estradiol and levonorgestrel in pharmaceutical dosage form. The developed method with mobile phase CHCl3: toluene: methanol (8:1:0.5 v/v/v), precoated silica gel on aluminum plate 60F254, (10 cm × 10 cm), used as stationary phase, chamber saturation time is 20 minutes. Detection was carried out at 230 nm UV detector. Rf value was found to be 0.4±0.004 and 0.6±0.007 for ethinyl estradiol and levonorgestrel respectively. The calibration curve of Ethinyl estradiol and Levonorgestrel was found to be linear in the range of 300-1800 ng/band and 1500-9000 ng/band respectively. The proposed method has been validated for precision, accuracy, robustness. Accuracy was performed by recovery studies at 80%, 100%, 120%. % recovery for ethinyl estradiol was found to be 99.03% - 99.96% and for levonorgestrel, it was 99.66% - 100.2%. LOD and LOQ values for ethinyl estradiol was 40.71 ng/band and 123.10 ng/band respectively and for levonorgestrel 397.03 ng/band and 1203.11 ng/band respectively. Precision of the method was assessed by performing repeatability, intra-day and inter-day precision studies. Thus, the developed method was found to be accurate, precise and relatively simple for the simultaneous estimation of ethinyl estradiol and levonorgestrel in pure form as well as in marketed formulation.
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